Intelligent biological probe microbiological detection method with stable fluorescence characteristic

• Main Authors: WAN YI, ZHONG YONGJIE, WU YOUJU, ZUO YONG
• Format: Patent
• Language: Chinese; English
• Published: 15.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides an intelligent biological probe microbiological detection method with stable fluorescence characteristics. The method comprises the following steps: S1, sampling; S2, preparing a detection solution: diluting a sample to be detected, and uniformly mixing to prepare a detection solution with a ratio of 1: 10; S3, culturing the detection solution: adding the culture solution into the detection solution in the step S2, and putting the detection solution into a constant-temperature incubator at 37 DEG C for culture; S4, preparing a working solution of the intelligent biological probe: adding the intelligent biological probe into a buffer solution according to a ratio of 1: 20000, and uniformly mixing; S5, culturing a probe solution: adding the detection solution in the step S3 into the working solution in the step S4, uniformly mixing to form the probe solution, and culturing; S6, marking the target cells: carrying out fluorescence marking on the target cells by the intelligent biological pr