OLIGONUCLEOTIDE FORMULATION METHOD

• Main Authors: MELDGAARD MICHAEL, APPELDORFF LARSEN INNA
• Format: Patent
• Language: Chinese; English
• Published: 01.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a method for determining the amount of an oligonucleotide present in a powdered form. The method does not require the experimental determination of the extinction coefficient of the oligonucleotide, or the serial dilution and A260 measurement. The method is, for example, applicable to modified oligonucleotides including 2' sugar modified oligonucleotides, phosphorothioate oligonucleotides, reporter labelled oligonucleotides and conjugated oligonucleotides. 本发明涉及一种用于确定以粉末形式存在的寡核苷酸的量的方法。所述方法不需要实验确定所述寡核苷酸的消光系数、或连续稀释和A260测定。所述方法例如适用于经修饰的寡核苷酸，包括2'糖修饰的寡核苷酸、硫代磷酸寡核苷酸、报告基因标记的寡核苷酸和缀合的寡核苷酸。