Method for preparing L-glufosinate-ammonium through double enzyme combination

• Main Authors: SUN BING, YI WANGYIN, HAN JINQUAN, PENG DA, WANG MINCHUN, WANG DONGCHAO
• Format: Patent
• Language: Chinese; English
• Published: 01.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses a method for preparing L-glufosinate-ammonium through double enzyme combination, which comprises the following steps: by taking 2-acetamido 4-[hydroxyl (methyl) phosphonyl]-DL-butyric acid or salt thereof as a raw material, carrying out hydrolysis reaction on a system formed by the raw material, anhydrous cobalt chloride and water, L-acetyl amino acid hydrolase and acetyl amino acid racemase under a certain pH condition to obtain an L-glufosinate-ammonium aqueous solution; treating an L-glufosinate-ammonium aqueous solution through a micro-filtration membrane to remove enzyme, treating the L-glufosinate-ammonium aqueous solution through a nano-filtration membrane to remove salt, adding ammonia water to adjust the pH value to 4-7, and sequentially concentrating and crystallizing with methanol to obtain the L-glufosinate-ammonium. according to the method, the two enzymes are commercialized, the commercialized codes of the L-acetyl amino acid hydrolase and the commercialized codes of the