Netted platelet detection dye liquor, detection reagent, preparation method of detection reagent, detection method of sample analyzer and sample analyzer

• Main Authors: LYU BIYU, ZHOU XIANBO, ZHENG JIANTONG, WU ZHONGFEN
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a netted platelet detection dye liquor, a detection reagent, a preparation method of the detection reagent, a detection method of a sample analyzer and the sample analyzer, and the provided nucleic acid dye can be used for a blood analyzer to meet the detection requirements of accurate and rapid counting of netted platelets. Mesh platelets are counted through reagent treatment, a specific mesh platelets nucleic acid dye and a mesh platelets cell membrane treatment reagent are added, and the mesh platelets can be accurately counted in a single channel, namely an RET channel, by adopting nucleic acid fluorescent staining and semiconductor laser flow cytometry. According to the method, the morphologies of reticulocytes and reticulated platelets are processed by a detection reagent, and the morphologies of various cell membranes are changed while the reticulocytes and the reticulated platelets are spheroidized, so nucleic acids of the reticulocytes and the reticulated platelets are respecti