Method for culturing grouper cells by using bioreactor

• Main Authors: YIN SHUNYI, REN ZHENGHUA, WU HUOLIAN, JIANG DONGYANG, YUAN CHUNYAN
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and particularly relates to a method for culturing grouper cells by using a bioreactor. The method provided by the invention comprises the following steps: culturing grouper cells on a large scale by virtue of microcarrier adherent suspension in a BC-7L bioreactor, and adding a GS cell growth solution, namely adding 50-80 ml of galactose and 100-160 ml of grouper tissue culture solution into a DMEM basic culture medium, so that metabolism of lower organisms can be promoted. According to the method provided by the invention, large-scale microcarrier culture of the grouper cells is realized, the yield of the microcarrier cells is high, the cultured cells are normal in morphology, the activity and hereditary characters of original cells are maintained, and the grouper cells have the capabilities of infecting and reproducing viruses and can be used for reproducing fish viruses and producing fish vaccines on a large scale. 本发明属于生物技术领域，具体涉及一种使用生物反应器培养石斑鱼细胞的方法