PCR (Polymerase Chain Reaction) primer, kit and method for identifying sex of pigeons

• Main Authors: FU SHENGYONG, GE QINGLIAN, LIU HONGXIANG, FAN YANFENG, TANG XIUJUN, JI GAIGE, ZHANG JING, GAO YUSHI, ZHANG XIAOYAN, MA YINPENG, HUANG SHENGHAI, JIA XIAOXU, GU RONG, LU JUNXIAN
• Format: Patent
• Language: Chinese; English
• Published: 17.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a PCR (Polymerase Chain Reaction) primer, a kit and a method for identifying the sex of pigeons. The sequence of the PCR primer for identifying the sex of the pigeons is shown as SEQ ID NO.1 and/or SEQ ID NO.2. The PCR primers are used for PCR amplification and agarose gel electrophoresis, the sex of ducks, chickens, pigeons, muscovy ducks, gooses or quails are determined according to the number of bands of electrophoresis results, the operation is simple and convenient, the identification result is rapid and accurate, the method is convenient for basic-level popularization and use, and the method has a wide market application prospect; and in addition, a detection kit developed based on the method provided by the invention can generate considerable economic benefits and good social values. 本发明提供一种用于鸽性别鉴定的PCR引物、试剂盒及其方法，所述鸽性别鉴定用PCR引物序列如SEQ ID NO.1和/SEQ ID NO.2所示。通过本发明的PCR引物进行PCR扩增和琼脂糖凝胶电泳，利用电泳结果的条带数来确定鸭、鸡、鸽、番鸭、鹅或鹌鹑的性别，具有操作简单方便，鉴别结果快速准确，便于基层推广和使用，具有广阔的市场应用前景，此外，基于本发明方法开发的检测试剂盒，可产生可观的经济效益和