Silkworm microsporidia milRNAs and application thereof

• Main Authors: HU CONGWU, LU CHENG, PAN MINHUI, DONG ZHANQI, ZHENG NING, CHEN PENG
• Format: Patent
• Language: Chinese; English
• Published: 17.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses silkworm microsporidia milRNAs and application thereof, research finds that milRNA-4, milRNA-8, milRNA-10 and milRNA-12 have a promoting effect on the proliferation of microsporidia, milRNA-1, milRNA-3 and milRNA-11 have an inhibiting effect on the proliferation of microsporidia, and the milRNA-8 has the most obvious promoting effect on the proliferation of microsporidia, so that the milRNA-8 can be used for regulating the proliferation of microsporidia. The research also finds that the target gene of miRNAs is BmPEX16, and the copy number of the overexpressed silkworm microsporidia and the protein expression quantity of the NbPTP2 are both reduced, which indicates that the proliferation of the silkworm microsporidia is inhibited after the overexpression of the BmPEX16; after the BmPEX16 is knocked out, the proliferation of the silkworm microsporidia is promoted, so that the proliferation of the silkworm microsporidia can be regulated by regulating the expression of the BmPEX16 gene, a