Method for rapidly detecting genetic sex of channel catfish based on SNP locus specific primer extension reaction

• Main Authors: WANG MINGHUA, DUAN YONGQIANG, BIAN WENJI, ZHANG SHIYONG, XU SIQI, ZHONG LIQIANG, CHEN XIAOHUI
• Format: Patent
• Language: Chinese; English
• Published: 10.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for rapidly detecting genetic sex of channel catfish based on SNP locus specific primer extension reaction, which comprises the following steps: designing a peripheral primer, a specific extension primer (ASE) and a DNA probe (ASE-DP) for amplifying sex-linked SNP loci of channel catfish, carrying out extension reaction and nucleic acid hybridization by utilizing the DNA, the primer and the DNA probe of a channel catfish to be detected, and judging the genetic sex of the to-be-detected sample according to the color development result of a rapid nucleic acid detection test strip. The accuracy of the method reaches 100%, and the method has a good application prospect. 本发明公开了一种基于SNP位点特异性引物延伸反应快速检测斑点叉尾鮰遗传性别的方法，该方法设计扩增斑点叉尾鮰性别连锁SNP位点的外围引物、特异性延伸引物(ASE)和DNA探针(ASE-DP)，利用所述待测斑点叉尾鮰的DNA、引物和DNA探针进行延伸反应和核酸杂交，再根据快速核酸检测试纸条显色结果判断待测样本的遗传性别。本发明的准确率达100％，具有很好的应用前景。