High performance liquid chromatography analysis method of traditional Chinese medicine reference substance ferulic acid

• Main Author: TAN XIAOYONG
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a high performance liquid chromatography analysis method of a traditional Chinese medicine reference substance ferulic acid, and relates to the technical field of analysis, and the method comprises the following steps: 1, preparing a ferulic acid sample into a ferulic acid sample solution with a concentration of 100-300 [mu]g/ml by using methanol; 2, detecting the ferulic acid sample solution by using a high performance liquid chromatograph; the detection conditions being as follows: a chromatographic column is a C18 chromatographic column; a mobile phase A being a trifluoroacetic acid aqueous solution, and the volume fraction being 0.005%-0.03%; the mobile phase B being chromatographic grade acetonitrile; the volume ratio of the mobile phase A to the mobile phase B being (80: 20)-(85: 15); and the detection wavelength being 324nm. The high performance liquid phase detection method of ferulic acid is not limited to a single condition factor, all detection conditions are systematically i