Method for detecting DNA/protein by MALDI MS based on BCA technology and taking Au NP as matrix

• Main Authors: TANG ZICHAO, CHEN YUWAN, JIANG YIHUANG
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a method for detecting DNA/protein by MALDI MS based on a BCA technology and taking AuNP as a matrix, and particularly relates to a novel analysis and detection method for performing MALDI MS detection on target DNA or target protein by taking mercaptide as a bar code and gold nanoparticles as a matrix based on a bio-bar code amplification method BCA (Bio-bar-code amplification). The method has the characteristics of high sensitivity, high specificity, high ionization efficiency, simplicity in operation and the like, and has a wide application prospect. 本发明公开了一种基于BCA技术以AuNP为基质的MALDI MS检测DNA/蛋白质的方法，具体方法是基于生物条形码扩增方法BCA(bio-bar-code amplification)，采用硫醇化物为条形码，采用金纳米颗粒为基质对目标DNA或目标蛋白质进行MALDI MS检测的一种新型分析检测方法。该方法具有高灵敏度、高特异性、高电离效率、操作简易等特点，应用前景广阔。