Method for detecting DNA/protein by MALDI MS based on BCA technology and taking Au NP as matrix
The invention discloses a method for detecting DNA/protein by MALDI MS based on a BCA technology and taking AuNP as a matrix, and particularly relates to a novel analysis and detection method for performing MALDI MS detection on target DNA or target protein by taking mercaptide as a bar code and gol...
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Main Authors | , , |
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Format | Patent |
Language | Chinese English |
Published |
07.09.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a method for detecting DNA/protein by MALDI MS based on a BCA technology and taking AuNP as a matrix, and particularly relates to a novel analysis and detection method for performing MALDI MS detection on target DNA or target protein by taking mercaptide as a bar code and gold nanoparticles as a matrix based on a bio-bar code amplification method BCA (Bio-bar-code amplification). The method has the characteristics of high sensitivity, high specificity, high ionization efficiency, simplicity in operation and the like, and has a wide application prospect.
本发明公开了一种基于BCA技术以AuNP为基质的MALDI MS检测DNA/蛋白质的方法,具体方法是基于生物条形码扩增方法BCA(bio-bar-code amplification),采用硫醇化物为条形码,采用金纳米颗粒为基质对目标DNA或目标蛋白质进行MALDI MS检测的一种新型分析检测方法。该方法具有高灵敏度、高特异性、高电离效率、操作简易等特点,应用前景广阔。 |
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Bibliography: | Application Number: CN202110441756 |