Preparation method and application of humanized ACE2 gene modified mouse embryonic stem cell model

• Main Authors: SUN JIAOYANG, KUANG JUNQI, WU GUANGMING, WU KAIXIN, QUAN XIONGZHI, CHEN JIEKAI, GUO LIN
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the field of biological medicines, and particularly relates to a preparation method and application of a humanized ACE2 gene modified mouse embryonic stem cell model. The invention provides an sgRNA fragment sequence. A targeting vector containing the sgRNA fragment sequence can specifically target an ACE2 gene and can be used for preparing the humanized ACE2 gene modified mouse embryonic stem cell model. The targeting specificity of the sgRNA fragment sequence and the efficiency of cutting a target gene are both good. 本发明涉及生物医药领域，尤其涉及一种人源化ACE2基因改造小鼠胚胎干细胞模型的制备方法及应用。本发明提供了一种sgRNA片段序列。含有这种sgRNA片段序列的靶向载体，能够特异性的靶向ACE2基因，可用于制备人源化ACE2基因改造小鼠胚胎干细胞模型。本发明的sgRNA片段序列的靶向特异性和切割目的基因的效率都较好。