Human-derived chordoma bone in-situ PDX model construction method and application thereof
The invention relates to the technical field of medical engineering, and particularly relates to a human-derived chordoma bone in-situ PDX model construction method and application thereof. The human-derived chordoma bone in-situ PDX model construction method comprises the steps that (1), chordoma p...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
07.09.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to the technical field of medical engineering, and particularly relates to a human-derived chordoma bone in-situ PDX model construction method and application thereof. The human-derived chordoma bone in-situ PDX model construction method comprises the steps that (1), chordoma patient tissue is selected; (2), the chordoma tissue is divided into three parts, one part is used for transcriptome sequencing, the other part is used for making a tissue chip, and the last part is implanted into the tibia medullary cavity of an immunodeficiency mouse; (3), the growth condition of the tibia tumor of the mouse is observed; (4), passage is conducted; and (5), the effectiveness and feasibility of the method are judged, and the method is used for detecting curative effect prediction and curative effect monitoring of potential new drugs. The method has the advantages that a model obtained through a chordoma construction method in the prior art cannot well simulate the disease characteristics and cannot |
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Bibliography: | Application Number: CN202110638354 |