Induction medium and induction method for inducing differentiation of human-derived induced multifunctional stem cells into mesenchymal stem cells

• Main Authors: QIN XINYUAN, YAO QI, DING LIXIANG, CAO YULIN, WANG JIANGNING, LI TIANBO, WANG JING, WANG SHUO, YU ZEYANG, GAO LEI, LIU HUANNING, YIN YEFENG, ZUO YOUWEI, LIU LIHUA
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a human induction culture medium and culture method for differentiating human induced multifunctional cells into mesenchymal stem cells. The human induction culture medium comprises a basic culture medium, and serum and an additive which are added into the basic culture medium, the serum is a serum-free substitute with the content of 10%, and the additive comprises the following components by content: 10% of a serum-free substitute, 5%-20% of calf serum, 20 ng/mL of basic fibroblast growth factors, 50 microgram/mL of ascorbic acid, 1% of 1x insulin transferrin selenium, 4.5mM of thioglycerol and 1% of non-essential amino acids. According to the induction culture medium and the induction method provided by the invention, the induced multifunctional stem cells can be infinitely expanded and can be easily converted into the mesenchymal stem cells derived from the induced multifunctional stem cells within 20 days. 本发明提供了一种人诱导多功能细胞分化成间充质干细胞的人诱导培养基及培养方法，人诱导培养基包括基础培养基与添加于所述基础培养基中的血清和添加剂，所述血清为含