Preparation method of electrochemical sensor for detecting ractopamine based on silica sol

• Main Authors: YUAN ZENGJIN, WENG JINGZHENG
• Format: Patent
• Language: Chinese; English
• Published: 03.09.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a preparation method of an electrochemical sensor for detecting ractopamine based on silica sol. The method comprises the following steps: mixing reactants of a component A into a three-neck bottle according to mass parts, heating, adding a component B, stirring, standing, stirring and cooling to 60 DEG C to obtain silica sol; mixing the component C into a flask, blending to obtain a graphene suspension, and carrying out ultrasonic dispersion to obtain a graphene aqueous solution; and sequentially polishing a platinum sheet by using an Al2O3 powder suspension, ultrasonically cleaning to drop silica sol on the surface of the platinum sheet, drying, dropwise adding a graphene aqueous solution, and dropwise adding a ractopamine antibody to obtain the ractopamine electrochemical sensor. The sensor constructed by the invention is applied to detection of a target component ractopamine in an actual sample, and the actual concentration range of detection is 0.1-1.0*10 ng/g, which is one order