EXTRACELLULAR VESICLES FOR TARGETED THERAPIES AGAINST MYELOID-DERIVED SUPPRESSOR CELLS

• Main Authors: GALLEGO-PEREZ DANIEL, DUARTE SANMIGUEL SILVIA, HIGUITA-CASTRO NATALIA, CARSON WILLIAM
• Format: Patent
• Language: Chinese; English
• Published: 20.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Disclosed herein are MDSC-targeted extracellular vesicles (EVs) loaded with therapeutic cargo, as well as compositions, systems, and methods for making same. Also disclosed herein is an MDSC-targeting ligand, such as a fusion protein containing an MDSC-targeting moiety. Also disclosed are EVs containing the disclosed fusion protein. In some embodiments, the EV is also loaded with a therapeutic cargo. Also disclosed is an EV-producing cell engineered to produce the disclosed EVs. Also disclosed is a method for making the disclosed EVs that involves culturing the disclosed EV-producing cells under conditions suitable to produce EVs. 本文公开了负载有治疗性货物的MDSC靶向的细胞外囊泡(EV)，以及其组合物、系统和制备方法。本文还公开了MDSC靶向配体，诸如含有MDSC靶向部分的融合蛋白。还公开了含有所公开的融合蛋白的EV。在一些实施方案中，EV还负载有治疗性货物。还公开了经工程改造以生产所公开的EV的EV生产细胞。还公开了制备所公开的EV的方法，所述方法涉及在适于生产EV的条件下培养所公开的EV生产细胞。