Separated carbohydrate binding domain specifically binding to kappa-carrageenan and application thereof

• Main Authors: XING MENGDAN, LIU GUANGLEI
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a separated carbohydrate binding domain PpCBM specifically binding to kappa-carrageenan. The amino acid sequence of the carbohydrate binding domain PpCBM is SEQ ID NO. 1. The structural domain PpCBM has no enzyme activity, but the specific enzyme activity of PpCgk can be remarkably improved through the combined action of the structural domain PpCBM and the kappa-carrageenan enzyme PpCgkCD, so that the structural domain PpCBM has higher substrate affinity and higher relative catalytic efficiency. The invention discloses a preparation method of the structural domain PpCBM and an application of the structural domain PpCBM in construction of engineering strains and degradation of kappa-carrageenan. According to the kappa-carrageenan engineering enzyme constructed by adopting the structural domain PpCBM, the hydrolytic activity is remarkably enhanced, the temperature stability is improved, the hydrolytic mode is limited for progressive hydrolysis, and the kappa-carrageenan engineering enzyme