DNA probe library for hybridization with 1, 2 or 3 genes of FGFR and application

• Main Authors: FAN JIA, YI YUTING, HUANG XIAOYONG, GUAN YANFANG, SHI GUOMING, HAO SHIGUANG, ZHOU JIAN, YI XIN, CHU YUXING, LI QINGLIN, SHENG KE, FU XIAORUI
• Format: Patent
• Language: Chinese; English
• Published: 13.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a DNA (Deoxyribose Nucleic Acid) probe library for hybridization with 1, 2 or 3 genes of a fibroblast growth factor receptor (FGFR) and application, and belongs to the technical field of biological medicines. According to the DNA probe library for hybridization with the FGFR1 gene, FGFR2 gene or FGFR3 gene provided by the invention, the DNA probes comprise all probes listed in SEQ ID NO.1-186. The invention provides a method for enriching FGFR1, FGFR2 or FGFR3 gene segments by utilizing the DNA probe library and detecting related fusion variation by combining with a next generation sequencing (NGS) technology. By adopting the gene enrichment method and the specific DNA probe library obtained by screening, fusion variation in the FGFR1, FGFR2 or FGFR3 genes can be accurately obtained by applying the next-generation sequencing technology. The probe library has the advantages of large detection flux, high accuracy, short period and high sensitivity. 本发明涉及一种用于与成纤维细胞生长因子受体(Fibroblast growt