Enhancer in colorectal cancer cells and application thereof

• Main Authors: XIE XINA, LIN JIADIAN
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the field of biology, in particular to an enhancer in colorectal cancer cells and application of the enhancer. The invention discloses an enhancer closely related to colorectal cancer cells. The enhancer can regulate proliferation of the colorectal cancer cells. The expression of eRNA obtained by transcription of the enhancer in colorectal cancer cells and tissues is relatively high, especially in RKO, HT29, HCT116, LoVo and SW480. According to the present invention, the specific enhancer fragment in the colorectal cancer cell is knocked out, or the transcription of the specific enhancer fragment is inhibited to obtain the eRNA, or the eRNA is knocked down, such that the multi-dimensional colorectal cancer cell proliferation inhibition method is provided so as to provide the technical idea for the research of the related drugs for preventing and treating the colorectal cancer. 本发明涉及生物领域，具体涉及结直肠癌细胞中的增强子及其应用。本发明公开了一个与结直肠癌细胞有着密切关系的增强子，该增强子可以调控结直肠癌细胞的增殖；该增强子转录得到的eRNA在结直肠癌细胞和组织中表达较高，特别是在RK