Anti-PD-1 antibody and application thereof

• Main Authors: LI FENG, ZHANG WENJUN, TIAN WENWU, TAN FANGHUI, WEI XUEMEI
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2021
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention belongs to the field of oncotherapy and molecular immunology, and provides an anti-PD-1 antibody and application thereof. The antibody is prepared by the following steps: immunizing a mouse with a human PD-1 antigen, constructing an antibody library and screening to obtain a mouse anti-human PD-1 antibody, and carrying out humanization and affinity maturation to obtain the high-affinity anti-PD-1 monoclonal antibody. The monoclonal antibody disclosed by the invention can be well and specifically combined with PD-1, immunosuppression of PD-1 on an organism is specifically relieved, and T lymphocytes are activated. 本发明属于肿瘤治疗和分子免疫学领域，提供了一种抗PD-1抗体及其应用。本发明通过人PD-1抗原免疫小鼠，构建抗体文库及筛选，获得鼠源抗人PD-1抗体，而后经过人源化及亲和力成熟后获得高亲和力的抗PD-1单克隆抗体。本发明所述单克隆抗体能够很好地特异性与PD-1结合，特异地解除PD-1对机体免疫抑制，激活T淋巴细胞。