DILUTION TAGGING FOR QUANTIFICATION OF BIOLOGICAL TARGETS
Embodiments of a method for accurate determination of biological target abundance can include generating a first set of molecules associated with a target sequence, where the first set of molecules includes a first set of dilution tags associated with a relative concentration profile; generating a s...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
06.08.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Embodiments of a method for accurate determination of biological target abundance can include generating a first set of molecules associated with a target sequence, where the first set of molecules includes a first set of dilution tags associated with a relative concentration profile; generating a second set of molecules including a second set of dilution tags associated with the first set of dilution tags; generating a dilution tagged mixture; amplifying the subsets of dilution tagged genetic targets using the second set of molecules; generating a modified dilution tagged mixture from the amplified subsets; determining, for the biological sample, a count of the distinct molecules including the target sequence; and/or determining, for the biological sample, an assessment of relative concentrations distinct species, such as over a vast dynamic range.
用于准确确定生物靶丰度的方法的实施方案可以包括生成与靶序列相关的分子的第一集合,其中所述分子的第一集合包括与相对浓度分布相关的稀释标签的第一集合;生成分子的第二集合,所述分子的第二集合包括与稀释标签的第一集合相关的稀释标签的第二集合;生成加稀释标签的混合物;使用分子的第二集合扩增加稀释标签的遗传靶的子集;从经扩增的子集生成 |
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Bibliography: | Application Number: CN201980065828 |