Purification method of bovine type I collagen

The invention relates to a purification method of bovine type I collagen, which comprises the following steps: dissolving bovine type I collagen subjected to crude extraction treatment in an acid solution, degrading by using proteolytic enzyme of non-collagenase, and monitoring the viscosity to cont...

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Bibliographic Details
Main Authors CHEN JINGHUA, XU SHUQIN, MAO BAOLIANG
Format Patent
LanguageChinese
English
Published 03.08.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a purification method of bovine type I collagen, which comprises the following steps: dissolving bovine type I collagen subjected to crude extraction treatment in an acid solution, degrading by using proteolytic enzyme of non-collagenase, and monitoring the viscosity to control the enzymolysis degree; adjusting the pH value of the supernate to 3.5-5, sequentially dropwise adding alcoholic solutions with the final concentrations of 15-25% v/v, 30-45% v/v, 50-70% v/v and 75-95% v/v, separating out four telopeptide-removed bovine type I collagen, centrifuging, redissolving in an acid solution, adjusting to an isoelectric point, centrifuging to take precipitate, redissolving in the acid solution again, dialyzing in the acid solution with the pH value of 3-5, filtering to remove bacteria, taking filtrate, and freeze-drying, and obtaining the purified bovine type I collagen. The bovine type I collagen provided by the invention has high purity and no immunogenicity, is in a sterile active fo
Bibliography:Application Number: CN202110685663