Saccharomyces cerevisiae expressed long-acting recombinant human bFGF-HSA fusion protein and preparation method of standard substance thereof

• Main Authors: XU GAOTAO, KONG GUOQING, ZHAO JUN, ZHOU WEI, LIU JIALU, HE ZHIYUAN, FAN YUFANG
• Format: Patent
• Language: Chinese; English
• Published: 03.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and particularly relates to a preparation method of saccharomyces cerevisiae expressed long-acting recombinant human bFGF-HSA fusion protein, which comprises the following steps: (1) constructing a saccharomyces cerevisiae secretory expression vector pYES2/CT-MF alpha-bFGF-HSA; (2) preparing and transforming of bFGF-HSA fusion protein engineering bacteria; and (3) carrying out induced expression and purification on the INVSc1/pYES2/CT-MF alpha-bFGF-HSA engineering bacteria. Meanwhile, the invention also provides a preparation method of the long-acting recombinant human bFGF-HSA fusion protein standard substance expressed by saccharomyces cerevisiae. The recombinant protein (bFGF-HSA) formed by fusing long-acting recombinant human bFGF expressed by saccharomyces cerevisiae and HSA is simple in production process, low in cost and uniform in product. 本发明属于生物技术领域，具体涉及一种酿酒酵母表达长效重组人bFGF-HSA融合蛋白的制备方法，包括以下步骤：(1)酿酒酵母分泌表达载体pYES2/CT-MFα-bFGF-HSA的构建；(2)bFGF-HSA融合蛋白