Preparation method of high-purity astaxanthin ester from haematococcus pluvialis

• Main Authors: LI SHUNYOU, WANG XINGYONG, CHANG RUYUN, ZHANG YONG, SHEN JIAHUI, JIANG HUILIN, WANG DENGLIANG
• Format: Patent
• Language: Chinese; English
• Published: 30.07.2021
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; SPIRITS; VINEGAR; WINE

The invention provides a preparation method of high-purity astaxanthin ester from haematococcus pluvialis, and relates to the technical field of astaxanthin ester processing. The preparation method disclosed by the invention comprises the following steps: carrying out homogenization wall-breaking treatment on fresh haematococcus pluvialis, and then adding lipase for enzymolysis; and heating and extracting the enzymolysis product by adopting an ethanol solution, concentrating an extracting solution, conducting dissolving by adopting a weak polar solvent, conducting adsorbing by adopting an adsorbent, conducting drying, conducting packing, adopting petroleum ether/ethyl acetate mixed liquor with different volume ratios as an eluent, carrying out gradient elution, collecting the eluent in sections, and conducting concentrating to respectively obtain the high-purity astaxanthin ester. The method is easy to operate, astaxanthin ester in haematococcus pluvialis can be efficiently extracted, and the obtained astaxan