Rabbit-derived anti-porcine parvovirus type 6 VP2 protein antibody and preparation method thereof

The invention discloses a rabbit-derived anti-porcine parvovirus type 6 VP2 protein antibody and a preparation method thereof. The preparation method comprises the following steps: designing a pair of specific primers according to a PPV6 epidemic strain genome sequence published by GenBank, construc...

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Main Authors DAI JUNFEI, DENG SHUMING, OU YUNWEN, ZHANG JIE, SHI YICHENG, WANG QIN, REN SHAOKE, PAN QIN, LIU LIJUN
Format Patent
LanguageChinese
English
Published 23.07.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a rabbit-derived anti-porcine parvovirus type 6 VP2 protein antibody and a preparation method thereof. The preparation method comprises the following steps: designing a pair of specific primers according to a PPV6 epidemic strain genome sequence published by GenBank, constructing a pET30a-PPV6-VP2 recombinant expression vector, carrying out IPTG induced expression, Ni-NTA resin affinity chromatography purification and urea dialysis renaturation at different gradients, and performing uniform mixing and emulsification with a Freund's adjuvant in a ratio of 1: 1; and preparing PPV6 recombinant VP2 protein immunogen. A New Zealand white rabbit is subjected to multi-point subcutaneous injection on the back, hyperimmune serum is prepared, and the antibody is purified by adopting a saturated (NH4)2SO4 salting-out method. The prepared rabbit-derived anti-porcine parvovirus type 6 VP2 protein antibody is has good purity without bacteria, mycoplasma or exogenous virus pollution, and has good spe
Bibliography:Application Number: CN202110426713