SCAFFOLD PROTEINS DERIVED FROM PLANT CYSTATINS

• Main Authors: MACPHERSON MIKE B, TOMLINSON DARREN
• Format: Patent
• Language: Chinese; English
• Published: 23.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to scaffold proteins derived from plant cystatins and to nucleic acids encoding them. The scaffolds are highly stable and have the ability to display peptides. The scaffolds are particularly well suited for constructing libraries, e.g. in phage display or related systems. The invention also related to various uses of the scaffolds, including in therapy, diagnosis, environmental and security monitoring, synthetic biology and research, and to cells and cell cultures expressing the scaffold proteins. 本发明涉及源自植物半胱氨酸蛋白酶抑制剂的支架蛋白以及编码它们的核酸。支架高度稳定并且具有展示肽的能力。支架尤其适于例如在噬菌体展示或相关的系统中构建文库。本发明也涉及支架的各种用途，包括在治疗、诊断、环境和安全性监测、合成生物学和研究中的用途，和涉及表达支架蛋白的细胞和细胞培养物。