Solid-liquid separation method of microbial culture solution

• Main Authors: JIANG WEIMING, LIU HUIQING, WANG XINBO, WANG ZHUYIN, WANG QINQIN, GONG HUANZHANG, LI XIANGQUN, ZHANG ZENGYUAN
• Format: Patent
• Language: Chinese; English
• Published: 16.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a solid-liquid separation method of a microbial culture solution, and belongs to the technical field of microbial fermentation separation. The method mainly comprises the steps of adding a solid-liquid separation accelerant into a microbial culture solution, performing uniform mixing, standing the mixture for 1-30 minutes, and then filtering or centrifuging to obtain microbial cells, wherein the solid-liquid separation accelerant is one or a mixture of more of polyacrylic acid, polyacrylamide, cationic polyacrylamide, a cationic polyacrylic acid derivative, polylysine, polyvinylamine, polyvinylpyrrolidone, chitosan, polyquaternium and biological polysaccharides. The method is has simple operation and high separation efficiency, is compatible with a downstream process, and is convenient for separation and purification of subsequent products. 本发明提供了一种微生物培养液的固液分离方法，属于微生物发酵分离技术领域。本发名主要方法如下：向微生物培养液中加入固液分离促进剂，混合均匀，放置1～30min，然后过滤或离心获得微生物细胞；所述的固液分离促进剂为聚丙烯酸、聚丙烯酰胺、阳离子聚丙烯酰胺、阳离子聚丙烯酸衍生物、聚赖氨酸、聚乙烯胺、聚乙