Fluorescent probe as well as preparation method and application thereof

• Main Authors: XIE XILEI, TANG BO, WANG XU, SUN YUANYU
• Format: Patent
• Language: Chinese; English
• Published: 06.07.2021
• Subjects: ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; TESTING

The invention belongs to the technical field of biological medicines, and particularly relates to a fluorescent probe as well as a preparation method and application thereof. The probe takes naphthalimide as a fluorescence signal unit and formamide as a peroxynitrite response group, and is high in response speed, high in sensitivity and good in selectivity. Besides, pyridine cations in the structure of the probe enable the probe to be gathered in mitochondria in a targeted manner, benzyl chloride groups can form covalent linkage with sulfhydryl protein in the mitochondria, and then the probe is anchored in the mitochondria. Even if the membrane potential of the mitochondria is remarkably reduced or even completely disappears, the probe does not seep out of the mitochondria. Therefore, stable and accurate fluorescence imaging detection can be carried out on the peroxynitrite in the mitochondria. 本发明属于生物医药技术领域，具体涉及一种荧光探针及其制备方法与应用。该探针以萘酰亚胺为荧光信号单元，以甲酰胺作为过氧亚硝酸根响应基团，响应速度快，灵敏度高，选择性好。此外，探针结构中的吡啶阳离子使其能够靶向聚集于线粒体，苄基氯基团能