Recombinant serum type-4 fowl adenovirus for expressing serum type-8 fowl adenovirus spike protein and a preparation method thereof

• Main Authors: LU HAO, YE JIANQIANG, WANG WEIKANG, SHAO HONGXIA, LI TUOFAN, XIE QUAN, LIAN MINGJUN, QIN AIJIAN
• Format: Patent
• Language: Chinese; English
• Published: 02.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a recombinant serum type-4 fowl adenovirus for expressing serum type-8 fowl adenovirus spike protein and a preparation method thereof, and the principle and the core key technology of the invention are that sgRNA is used for replacing EGFP and then virus purification is carried out to obtain the recombinant fowl adenovirus capable of stably amplifying the fowl adenovirus type 8 spike protein. The recombinant FAdV-4 for expressing the fowl adenovirus type-8 spike protein is successfully obtained by inserting the fowl adenovirus type-8 spike protein gene F which is popular at present as a carrier, and an in-vitro experiment result shows that the replication capacity of the FAdV4-F1-F-F2 recombinant virus is almost the same as that of a FAdV-4 wild virus, and the recombinant virus can be used as an inactivated recombinant multivalent vaccine for preventing and controlling FAdV-4 and FAdV-8. 本发明涉及表达血清8型禽腺病毒纤突蛋白重组血清4型禽腺病毒及其制备方法，发明的原理和最核心的关键技术是利用sgRNA，替换EGFP后进行病毒纯化，获得可以稳定扩增血清8型禽腺病的纤突蛋白的重组禽腺