Synthesis of DNA with improved yield
The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large scale, with an improved yield and/or with an improved efficiency. The species of cation present in the nucleotide salt as the c...
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Main Authors | , , , , |
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Format | Patent |
Language | Chinese English |
Published |
29.06.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large scale, with an improved yield and/or with an improved efficiency. The species of cation present in the nucleotide salt as the counter-ion is critical to the yield, efficiency and fidelity of high yielding enzymatic DNA synthesis reaction. The processes herein use alternative cations as counter-ions for the ionic nucleotides, permitting the use of higher concentrations of nucleotides in DNA synthesis, and further allowing for more favourable reaction conditions to be used.
本发明涉及一种改进的合成脱氧核糖核酸(DNA)的方法,特别是无细胞酶促合成DNA,优选大规模地,该方法具有提高的产量和/或提高的效率。在核苷酸盐中作为抗衡离子存在的阳离子种类对于高产率酶促DNA合成反应的产量、效率和准确度至关重要。本文的方法使用替代性阳离子作为离子核苷酸的抗衡离子,从而允许在DNA合成中使用更高浓度的核苷酸,并进一步允许使用更有利的反应条件。 |
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Bibliography: | Application Number: CN201980067811 |