Synthesis of DNA with improved yield

• Main Authors: ADIE THOMAS ANTONY JAMES, ROTHWELL PAUL JAMES, PORTER NEIL, BABULA ANNA, GEHRIG-GIANNINI STEFANIE
• Format: Patent
• Language: Chinese; English
• Published: 29.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large scale, with an improved yield and/or with an improved efficiency. The species of cation present in the nucleotide salt as the counter-ion is critical to the yield, efficiency and fidelity of high yielding enzymatic DNA synthesis reaction. The processes herein use alternative cations as counter-ions for the ionic nucleotides, permitting the use of higher concentrations of nucleotides in DNA synthesis, and further allowing for more favourable reaction conditions to be used. 本发明涉及一种改进的合成脱氧核糖核酸(DNA)的方法，特别是无细胞酶促合成DNA，优选大规模地，该方法具有提高的产量和/或提高的效率。在核苷酸盐中作为抗衡离子存在的阳离子种类对于高产率酶促DNA合成反应的产量、效率和准确度至关重要。本文的方法使用替代性阳离子作为离子核苷酸的抗衡离子，从而允许在DNA合成中使用更高浓度的核苷酸，并进一步允许使用更有利的反应条件。