Method for detecting content of astaxanthin in astaxanthin emulsion in water and cosmetics of astaxanthin emulsion in water

• Main Authors: CAO PEIXIN, MEN JINQI, REN SIWEI, ZHENG YUBIN, LI YUANYUAN
• Format: Patent
• Language: Chinese; English
• Published: 22.06.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting the content of astaxanthin in an astaxanthin emulsion in water and cosmetics of the astaxanthin emulsion in water. The method compriss the following steps: adding water, uniformly mixing, adding methanol, uniformly mixing, adding dichloromethane, uniformly mixing, standing, centrifuging, taking a lower organic phase, and diluting the organic phase with acetone until the absorbance value is 0.2-0.8 under 474 nm; carrying out enzymolysis on the organic phase diluted by acetone by using cholesterol esterase to obtain enzymatic hydrolysate, extracting the enzymatic hydrolysate by using petroleum ether, collecting a petroleum ether layer, blow-drying by using nitrogen, and redissolving by using a mixed solution of n-hexane and acetone to obtain a sample solution of the sample to be detected; detecting a pre-prepared astaxanthin standard solution by using a high performance liquid chromatography, determining a standard curve, then detecting a sample solution of the sam