Heat-resistant beta-mannase mutant ManAK-5 as well as coding gene and application thereof

• Main Authors: ZHANG FANG, MOU HAIJIN, YUAN MINGXUE, LIU ZHEMIN
• Format: Patent
• Language: Chinese; English
• Published: 22.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention relates to a heat-resistant beta-mannase mutant ManAK-5 as well as a coding gene and application thereof, and belongs to the field of gene engineering and enzyme engineering, the mutant is mutated on the basis of an amino acid sequence of wild beta-mannase ManAK as shown in SEQ ID NO.1, and a mutation site is selected from one of G331C, S158Y, Q166W, S177R, S180I, K99W, N178R, N178I, D273C/V308C or N277C/T315C. The heat-resistant stability of the mutant disclosed by the invention is improved, and the wide pH and the optimal temperature of wild beta-mannase ManAK are basically kept. 本发明涉及一种耐热性β-甘露聚糖酶突变体ManAK-5及其编码基因和应用，属于基因工程和酶工程领域，所述突变体是在野生β-甘露聚糖酶ManAK的氨基酸序列如SEQ ID NO.1所示基础上进行的突变，所述的突变位点选自G331C、S158Y、Q166W、S177R、S180I、K99W、N178R、N178I、D273C/V308C或N277C/T315C中的一种。本发明突变体的耐热稳定性提高，且基本保持了野生β-甘露聚糖酶ManAK的的广泛pH和最适温度。