Modified transposases for improved insertion sequence bias and increased DNA input tolerance

The application relates to modified transposases for improved insertion sequence bias and increased DNA input tolerance. Provided herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction con...

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Main Authors OSOTHPRAROP TRINA FAYE, GLOECKNER CHRISTIAN, GRUNENWALD HAIYING LI, SCHLINGMAN DANIEL, VAIDYANATHAN RAMESH, BOMATI ERIN, HE MOLLY, HASKING DARIN, BURGESS JOSHUA, KIA AMIRALI, KHANNA ANUPAMA, KUERSTEN SCOTT
Format Patent
LanguageChinese
English
Published 22.06.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The application relates to modified transposases for improved insertion sequence bias and increased DNA input tolerance. Provided herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same. 本申请涉及用于改进插入序列偏倚和增加DNA输入耐受性的修饰的转座酶。本文提供了用于改进核酸样品的片段化和加标签的转座酶和反应条件，特别地表现出改进的插入序列偏倚的改变的转座酶和反应条件，以及使用以上描述的转座酶和反应条件的方法及试剂盒。
Bibliography:Application Number: CN202110295229