Recombinant serum 4 type fowl adenovirus with green fluorescent protein replacing Fiber-2 and preparation method of recombinant serum 4 type fowl adenovirus

• Main Authors: WAN ZHIMIN, YE JIANQIANG, SHAO HONGXIA, LI TUOFAN, XIE QUAN, MU YARU, QIN AIJIAN
• Format: Patent
• Language: Chinese; English
• Published: 22.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a recombinant serum 4 type fowl adenovirus with Fiber-2 replaced by green fluorescent protein and a preparation method thereof, the present epidemic serum 4 type fowl adenovirus is used as a carrier, F2 which is one of virulence genes of the virus is deleted, and after purification, the F2 completely deleted recombinant FAdV4 virus for expressing EGFP is successfully obtained. In-vivo and in-vitro experimental results show that the virulence of the FAV4-EGFP-rF2 recombinant virus is remarkably reduced, and the protection rate after challenge is very good, so that the F2 complete deletion type recombinant virus provided by the invention not only can provide a biological material for prevention and control of FAdV-4, but also can be used as an insertion site for inserting other pathogen protective antigens by replacing the F2 position with EGFP, so that the FAV4-EGFP-rF2 recombinant virus can be used for preventing and controlling FAdV-4. And a solid theoretical basis is provided for co