Primers for identifying pathogenic microorgansims of apple tree branch and trunk rot and disease degree grading method

• Main Authors: YANG JIANGSHENG, XIE CHUNYANG, LI YIJING, MA WEICHAO
• Format: Patent
• Language: Chinese; English
• Published: 18.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of identification of pathogenic microorganisms, in particular toprimers for identifying pathogenic microorganisms of apple tree branch and trunk rot and a disease degree grading method. The identification primers have good specificity, can conduct specific amplification on genomic DNA of three strains of methylobacterium brachiatum, sphingomonas aquatilis and aureobasidium pullulans in robust fruit trees, two strains of valsa mali and botryococcus brauni in diseased fruit trees, and four strains of sagonosporopsis lupini, cladosporium allicum, didymella keratinophil and cladosporium delicatulum in withered fruit tree after disease, are free of primer dimers and non-specific amplification, can be used for detecting a diseased state and a diseased degree of apple tree branch rot in an incubation period with a good effect, further judges the diseased state and the diseased degree of the apple tree branch rot, helps establish a detection and grading standard of the dis