Method for targeted capture of hereditary hearing loss gene sequence and sequencing method

• Main Authors: JIAO YANG, JIA DANMEI, XU YAPING, CHEN QIONGQIONG, LIU YA, ZHANG JINGLAN, CAI LUHANG, JIA JUNLING
• Format: Patent
• Language: Chinese; English
• Published: 18.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biology, and particularly provides a method for targeted capture of a hereditary hearing loss gene sequence and a sequencing method. The method comprises the steps that a capture membrane for targeted capture of positive and negative chains of the hereditary hearing loss gene exon sequence is prepared, wherein hereditary hearing loss genes comprise CDH23, COL11A1, DSPP, MYO7A, OTOF, PCDH15, MT-RNR1, MT-TL1, MT-TS1, MYO15A, SLC26A4, DFNA5, GJB2, GJB3, KCNJ10, SOX10, TCOF1, WFS1, DFNB59, USH1G, TMC1 and DFNB31; a fragmented human genome sample is constructed into a nucleic acid library applied to high-throughput sequencing; denaturation treatment is conducted on the nucleic acid library, and the nucleic acid library subjected to denaturation treatment is hybridized with the capture membrane under hybridization conditions to form a target nucleic acid molecule-capture membrane compound; and the target nucleic acid molecule-capture membrane compound is cleaned by us