Exo-inulinase mutant MutV268delta13 with improved low-temperature adaptability

• Main Authors: ZHANG RUI, HUANG ZUNXI, LI JUNJUN, CEN XIAOLONG, ZHOU JUNPEI, GAO YANXIU, HAN NANYU, XU BO, WU QIAN, TANG XIANGHUA
• Format: Patent
• Language: Chinese; English
• Published: 18.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an exo-inulinase mutant MutV268delta13 with improved low-temperature adaptability, and the mutant MutV268delta13 has an amino acid sequence as shown in SEQ ID NO. 1. Compared with a wild enzyme InuAMN8, the thermal activity and the thermal stability of the mutant enzyme MutV268delta13 are changed, the activity at low temperature is improved, the activity and the stability at medium temperature are reduced, and the mutant enzyme MutV268delta13 is favorably applied to the technical field of biology under low-temperature environment requirements. The temperature is increased from 0 DEG C to 25 DEG C, the activity of the InuAMN8 is increased from 15% to 74%, and the activity of the MutV268delta13 is increased from 24% to 94%; after treatment at 50 DEG C, the activity of the InuAMN8 is kept at 81% or above, and the enzyme activity of the MutV268 delta 13 is reduced from 25% to 0%. The mutant MutV268 delta 13 can be applied to the industries of food, wine brewing, washing and the like. 本发明公开