Method for establishing neuroblastoma in-situ model

• Main Authors: LIU JIANJUN, NIE LULIN, REN XIAOHU, SUN QIAN
• Format: Patent
• Language: Chinese; English
• Published: 18.06.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for establishing a neuroblastoma in-situ model. The method comprises the steps: S10. mixing an MEM culture medium and an F12 culture medium according to a ratio of 1: 1 so as to prepare a culture medium mixed solution, inoculating the culture medium mixed solution with neuroblastoma, and carrying out cell culture; S20. diluting the cultured neuroblastoma, so as to prepare a cell suspension; and S30. taking and injecting the cell suspension to the periphery of a skull of a C57BL/6 mouse, culturing the C57BL/6 mouse, and determining that the neuroblastoma in-situ model is obtained when a volume k of the neuroblastoma is 2.2cm to 3.5cm . According to the method, eligible neuroblastoma models can be screened out, the accuracy of establishing the neuroblastoma models is improved, and later-stage research is better facilitated. 本发明公开一种神经母细胞瘤原位模型的建立方法，包括S10、按1:1比例将MEM培养基和F12培养基混合制成培养基混合液，将神经母细胞瘤接种于所述培养基混合液中进行细胞培养；S20、将培养后的神经母细胞瘤进行稀释，制成细胞悬液；S30、取用所述细胞悬液注入C57BL/6小鼠的颅骨周边，并将所述C57BL/6小鼠进行