IMPROVED HIGH-THROUGHPUT COMBINATORIAL GENETIC MODIFICATION SYSTEM AND OPTIMIZED CAS9 ENZYME VARIANTS

The present invention provides to an improved high-throughput system and method for generating and screening of genetic variants by combinatorial modifications. Also provided are optimized SpCas9 enzyme variants produced by this system. 本发明提供一种用于通过组合修饰产生和筛选基因变体的改进的高通量系统和方法。还提供由该系统产生的优化的SpCas9酶变体。...

Full description

Saved in:
Bibliographic Details
Main Authors CAI ZHENGZI, HUANG ZHAOLIN
Format Patent
LanguageChinese
English
Published 11.06.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMBINATORIAL CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The present invention provides to an improved high-throughput system and method for generating and screening of genetic variants by combinatorial modifications. Also provided are optimized SpCas9 enzyme variants produced by this system. 本发明提供一种用于通过组合修饰产生和筛选基因变体的改进的高通量系统和方法。还提供由该系统产生的优化的SpCas9酶变体。
Bibliography:Application Number: CN201980061793