Detection method for bovine SEPT7 gene insertion/deletion mutation and application thereof

• Main Authors: SHEN CHENGLONG, LI JIE, ZHANG SIHUAN, NIU ZHIHAN, TIAN XIN, LEI ZUO, LAN KANGSHU, PAN CHUANYING, GAO LONGXIN, WANG YONGSHENG, SONG ENLIANG, JIANG FUGUI, FEI PANFENG
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a detection method for bovine SEPT7 gene insertion/deletion mutation and application of the detection method. Bovine whole genome DNA is used as a template, a part of fragments of the bovine SEPT7 gene are amplified through PCR, and agarose gel electrophoresis is applied to identify the genotype of the 9-bp insertion mutation of the individual SEPT7 gene. According to the character association analysis result, the genotype of the SEPT7 gene 9-bp insertion mutation site is significantly related to the breeding character of the cow. Therefore, the SEPT7 gene 9-bp insertion mutation site can be used for early marker-assisted selection of cow reproductive traits so as to accelerate establishment of cow populations with excellent genetic resources. 本发明公开了一种牛SEPT7基因插入/缺失突变的检测方法及其应用。以牛全基因组DNA为模板，通过PCR扩增牛SEPT7基因部分片段，应用琼脂糖凝胶电泳鉴定个体SEPT7基因9-bp插入突变的基因型。根据性状关联分析结果，SEPT7基因9-bp插入突变位点的基因型与母牛的繁殖性状显著相关。因此，SEPT7基因9-bp插入突变位点可用于母牛繁殖性状的早期标记辅助选择，以加快建立遗传资源优良的牛群体。