Device for realizing exosome enrichment by using filter membrane and exosome enrichment method

• Main Authors: YUAN HUIMING, LI YILAN, ZHANG LIHUA, ZHANG YUKUI, DAI ZHONGPENG, YANG KAIGUANG
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a device for realizing exosome enrichment by using a filter membrane and application of the device, in particular to a device capable of quickly enriching exosomes in body fluid at high flux. The device is characterized in that the enrichment of the exosomes in the body fluid and the removal of interference components of the exosomes in the body fluid are realized by selecting the filter membrane with the aperture matched with the size of the exosomes according to a suction filtration principle. The device is simple to operate and large in treatment flux, the suction filtration speed can be controlled, and rapid enrichment of the exosome can be realized. The device can be applied to the acquisition of exosomes in plasma, urine, cell culture fluid and the like. 本发明涉及一种利用滤膜实现外泌体富集的装置及其应用，是一种可高通量，快速对体液中外泌体进行富集的装置。本发明的特点是利用抽滤原理选择与外泌体尺寸匹配的孔径的滤膜实现体液中外泌体的富集及其干扰成分的去除。该装置操作简单，处理通量大，可控制抽滤速度，可实现外泌体的快速富集。该装置可应用于血浆，尿液，细胞培养液等中的外泌体的获取。