Rapid detection method for cyanide in sorghum

• Main Authors: SU NING, LU CHUNLING, HAN YUNCUI, LYU ZHIYUAN, QIN BINGWEI, QIU ZHENQING, LI QIANG, MA CHUNLIAN
• Format: Patent
• Language: Chinese; English
• Published: 01.06.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the field of food analysis, and provides a rapid detection method of cyanide in sorghum. The method comprises the following steps of: directly adding a crushed sample into a headspace sample introduction bottle; ultrasonically extracting cyanide in sorghum by using ultrapure water; performing still standing in an ice-water bath for 5 minutes; adding a phosphate buffer solution and chloramine T under the condition of the ice-water bath, immediately performing covering and uniformly mixing; performing detection through gas chromatography-headspace chromatography, adopting a sample injection mode with the split ratio being 50: 1; performing quantification through using a standard curve method; and finally, calculating the content of the cyanide in the sorghum. According to the method, a chromatographic column used for gas chromatography-headspace chromatography detection is an HP-5 chromatographic column, and the specification of the chromatographic column is 30 m*0.32 mm*0.25 mu m; and