Fluorescence resonance energy transfer magnetic sensor for exosome detection and preparation method thereof

• Main Authors: ZHU NANXING, WU YAO, YI QIANGYING, LI GUOHAO
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a fluorescence resonance energy transfer magnetic sensor for exosome detection and a preparation method. The sensor comprises Fe @ QD @ Apt with fluorescence and DNA modified gold nanoparticles, and the Fe @ QD @ Apt with fluorescence and the DNA modified gold nanoparticles are assembled together through complementary pairing DNA and Apt. Based on a fluorescence energy resonance transfer technology, the detection method has the characteristics of convenience, rapidness, low price and ultralow detection limit, and provides an important thought for liquid biopsy application. 本发明公开了一种用于外泌体检测的荧光共振能量转移磁传感器及制备方法，该传感器包括带有荧光的Fe@QD@Apt和DNA修饰的金纳米粒子，该带有荧光的Fe@QD@Apt和DNA修饰的金纳米粒子通过互补配对DNA与Apt组装在一起。本发明基于荧光能量共振转移技术，检测方法具有方便快捷、价格低廉、超低检测限的特点，为液体活检应用提供重要思路。