Preparation method of core-shell nano polymer and application of core-shell nano polymer in cyclic regeneration of coenzyme

• Main Authors: SUN PENGXUE, YANG YIYU, WANG LE
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a preparation method of a core-shell nano polymer and application of the core-shell nano polymer in cyclic regeneration of coenzyme. The polymer with a core-shell structure is prepared by co-immobilizing a coenzyme polymer and two coenzyme dependent enzymes in situ through a metal framework compound ZIF material. According to the method, a ZIF shell is utilized to limit a multi-enzyme biological catalytic reaction area, and cyclic regeneration of coenzyme and double-enzyme cascade reaction are realized by utilizing double enzymes under the action of an initiator and a substrate respectively. During use of the core-shell polymer, no additional coenzyme needs to be added, and after the core-shell polymer is repeatedly used for five times, the ratio of the substrate conversion rate to the initial conversion rate is still 70-80%. Therefore, the core-shell nano polymer has a very wide prospect in biosynthesis and conversion in which coenzyme participates. 本发明公开了一种核壳纳米多聚物的制备方法及其在辅酶循环再生上的应用，通