Primer probe combination for detecting SARS-CoV-2 virus and application of primer probe combination

• Main Authors: SHI QINGXI, WANG LEILEI, YANG SHUTING, WANG ZHIWEI
• Format: Patent
• Language: Chinese; English
• Published: 25.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer probe combination for detecting SARS-CoV-2 virus and an application of the primer and probe combination. The primer probe combination for detecting the SARS-CoV-2 virus specifically amplifies and detects any one or a combination of at least two of an ORF1ab gene, an N gene or an E gene of the SARS-CoV-2 virus. The invention also provides an SARS-CoV-2 virus joint detection kit based on the microfluidic digital PCR and a use method of the SARS-CoV-2 virus joint detection kit. The primer probe combination for detecting the SARS-CoV-2 virus is good in specificity, high in sensitivity and accurate in detection result; the SARS-CoV-2 virus joint detection kit based on the microfluidic digital PCR is short in detection time consumption and relatively low in cost; the operation is simple, and the product can be used for qualitative detection and quantitative detection, and has extremely high application value. 本发明提供了一种检测SARS-CoV-2病毒的引物探针组合及其应用，所述检测SARS-CoV-2病毒的引物探针组合特异性扩增并检测SARS-CoV-2