Simple preparation method of rapid PCR template of filamentous fungi

• Main Authors: GUI YANLING, HAN JIE, TANG GUANGFU, ZHAO JIEHONG, MAN HAIQIAO
• Format: Patent
• Language: Chinese; English
• Published: 18.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a simple preparation method of a rapid PCR template of filamentous fungi. The simple preparation method comprises the following steps of: (1) culturing to-be-detected filamentous fungi to obtain mycelia; (2) picking the mycelia of the filamentous fungi and putting the mycelia into a glass test tube filled with distilled water to obtain a mycelia mixed solution; (3) carrying out ultrasonic treatment on the glass test tube containing the mycelia mixed solution; and (4) directly taking the mycelia mixed solution subjected to ultrasonic treatment as a DNA template to carry out PCR amplification. The method disclosed by the invention does not need liquid nitrogen grinding, does not need to add any extraction reagent, is simple and rapid, and saves time and labor. The defects that existing filamentous fungi are long in DNA extraction time, more in added reagent and more in required instrument are overcome. The DNA template extracted by the method disclosed by the invention can obtain a stabl