Murine single-chain antibody for resisting swine transmissible gastroenteritis virus and preparation method of murine single-chain antibody

• Main Authors: QU MINGSHENG, SHAN XUEQIN, WANG YANAN, GAO DONGMEI, FAN YUFANG
• Format: Patent
• Language: Chinese; English
• Published: 18.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a murine single-chain antibody for resisting swine transmissible gastroenteritis virus and a preparation method of the murine single-chain antibody. A heavy chain variable region and a light chain variable region of a mouse anti-TGEV monoclonal hybridoma cell amplification antibody are connected through a linker and cloned into a prokaryotic expression vector, wherein the amino acid sequence of the single-chain antibody is shown as SEQ ID No.2. The molecular weight of the single-chain antibody obtained through inducible expression and purification is about 28 kDa, ELISA shows that the single-chain antibody can specifically recognize the swine transmissible gastroenteritis virus; a pseudovirus neutralization test shows that the single-chain antibody can neutralize the capacity of TGEV pseudovirus infected PK15 cells, and the anti-TGEV single-chain antibody is expected to neutralize the infectivity of the virus in a piglet body, and infection and transmission of viruses in a piglet group