Method for in-vitro amplification and activation of natural killer cells and pharmaceutical composition of natural killer cells

• Main Authors: ZHANG SHUNLANG, YANG ZHIYA, LIN JIELIANG
• Format: Patent
• Language: Chinese; English
• Published: 11.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a method for in-vitro amplification and activation of natural killer cells. According to the method, a culture medium containing nattokinase is used for culturing the natural killer cells, an optimized culture environment is provided for the natural killer cells exclusively, the cell amplification multiple is effectively increased under the condition that cancer feeder cells are not used for co-culture, and the natural killer cells which are high in purity and have high toxic activity on cancer cells are generated. The invention further relates to a pharmaceutical composition for inhibiting tumor cell proliferation. The pharmaceutical composition comprises the natural killer cells. 本发明涉及一种体外扩增和活化自然杀伤细胞的方法，该方法以含有纳豆激酶的培养基进行自然杀伤细胞培养，专属针对自然杀伤细胞提供优化的培养环境，且在不使用癌饲养细胞共同培养的条件下，有效率地提升细胞扩增倍数，并产生高纯度及对癌细胞具有高度毒杀活性的自然杀伤细胞。本发明还涉及一种用于抑制肿瘤细胞增殖的药物组合物，其包含自然杀伤细胞。