PKEK fusion protein as well as preparation method and application thereof

• Main Authors: CAO CHUNLAI, XIAO BINGWEN, LIANG XIONGJI, HUANG YOUQUAN, YANG QI, YANG XIAOCHUN, HE XIUYI, LIU XUEMING, LIU HEDONG, WANG KANGZHAO, ZOU XIN, ZHOU CUI
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a PKEK fusion protein as well as a preparation method and application thereof. The fusion protein is formed by sequentially connecting a plurality of flexible linker peptides and PKEK in series; and the fusion protein is expressed in a microbial cell in the form of inclusion body protein, meanwhile, a plurality of flexible linker peptides and the whitening polypeptide PKEK are sequentially connected in series to form the fusion protein, sheared impurities generated in the enzyme digestion process can be effectively reduced, then the high-purity whitening polypeptide PKEK is efficiently obtained, and the purity can reach 95.4%. Besides, a large amount of PKEK fusion protein inclusion bodies can be obtained within 12 h by utilizing the preparation method for producing the whitening polypeptide PEKE by utilizing a genetic engineering technology, the highest expression quantity can reach 41.5 g/L, and the process is simple, efficient, easy to amplify and beneficial to industrial production