Method for removing pepsin endotoxin
The invention provides a method for removing pepsin endotoxin, and belongs to the technical field of pepsin. The method comprises the following steps of: 1) dissolving: dissolving a pepsin finished product with unqualified endotoxin content in water for injection, adding sodium chloride, stirring an...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
04.05.2021
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides a method for removing pepsin endotoxin, and belongs to the technical field of pepsin. The method comprises the following steps of: 1) dissolving: dissolving a pepsin finished product with unqualified endotoxin content in water for injection, adding sodium chloride, stirring and dissolving, and filtering and clarifying; 2) performing ultrafiltration: concentrating clear liquid obtained by filtering and clarifying by using an ultrafiltration membrane, and adding water for injection for repeated dialysis until no chloride ion exists; 3) sterilizing: sterilizing and filtering by adopting a filter membrane; and 4) performing freeze drying on the product to obtain a pepsin product without endotoxin. Compared with a common endotoxin removal method, the membrane separation method adopted by the invention can effectively protect the enzyme activity, has a more remarkable endotoxin removal effect, and can enable the endotoxin content in the pepsin product to reach the industrial standard.
本发明提供一种 |
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Bibliography: | Application Number: CN202011548954 |